A microbial profiling method for the human microbiota using high-throughput sequencing.

ABSTRACT: Study of the human microbiota in relation to human health and disease is a rapidly expanding field. To fully understand the complex relationship between the human gut microbiota and disease risks, study designs that capture the variation within and between human subjects at the population level are required, but this has been hampered by the lack of cost-effective methods to characterize this variation. Illumina sequencing is inexpensive and produces millions of reads per run, but it is unclear whether short reads can adequately represent the microbial community of a human host. In this study, we examined the utility of a profiling method, microbial nucleotide signatures (MNS), focused on low-depth sampling of the human microbiota using Ilumina short reads. This method is intended to aid i

SUBMITTER: Tseng HH 

PROVIDER: S-EPMC3764493 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature