A baculovirus-mediated strategy for full-length plant virus coat protein expression and purification.

ABSTRACT:

Background

Garlic production is severely affected by virus infection, causing a decrease in productivity and quality. There are no virus-free cultivars and garlic-infecting viruses are difficult to purify, which make specific antibody production very laborious. Since high quality antisera against plant viruses are important tools for serological detection, we have developed a method to express and purify full-length plant virus coat proteins using baculovirus expression system and insects as bioreactors.

Results

In this work, we have fused the full-length coat protein (cp) gene from the Garlic Mite-borne Filamentous Virus (GarMbFV) to the 3'-end of the Polyhedrin (polh) gene of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV). The recombinant bac

SUBMITTER: Ardisson-Araujo DM 

PROVIDER: S-EPMC3765376 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature