HIV latency and integration site placement in five cell-based models.

ABSTRACT:

Background

HIV infection can be treated effectively with antiretroviral agents, but the persistence of a latent reservoir of integrated proviruses prevents eradication of HIV from infected individuals. The chromosomal environment of integrated proviruses has been proposed to influence HIV latency, but the determinants of transcriptional repression have not been fully clarified, and it is unclear whether the same molecular mechanisms drive latency in different cell culture models.

Results

Here we compare data from five different in vitro models of latency based on primary human T cells or a T cell line. Cells were infected in vitro and separated into fractions containing proviruses that were either expressed or silent/inducible, and integration site populations sequenced from

SUBMITTER: Sherrill-Mix S 

PROVIDER: S-EPMC3765678 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature