De novo sequencing of sunflower genome for SNP discovery using RAD (Restriction site Associated DNA) approach.

ABSTRACT:

Background

Application of Single Nucleotide Polymorphism (SNP) marker technology as a tool in sunflower breeding programs offers enormous potential to improve sunflower genetics, and facilitate faster release of sunflower hybrids to the market place. Through a National Sunflower Association (NSA) funded initiative, we report on the process of SNP discovery through reductive genome sequencing and local assembly of six diverse sunflower inbred lines that represent oil as well as confection types.

Results

A combination of Restriction site Associated DNA Sequencing (RAD-Seq) protocols and Illumina paired-end sequencing chemistry generated high quality 89.4 M paired end reads from the six lines which represent 5.3 GB of the sequencing data. Raw reads from the sunflower line, RHA

SUBMITTER: Pegadaraju V 

PROVIDER: S-EPMC3765701 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature