Characterization of constitutive CTCF/cohesin loci: a possible role in establishing topological domains in mammalian genomes.

ABSTRACT:

Background

Recent studies suggested that human/mammalian genomes are divided into large, discrete domains that are units of chromosome organization. CTCF, a CCCTC binding factor, has a diverse role in genome regulation including transcriptional regulation, chromosome-boundary insulation, DNA replication, and chromatin packaging. It remains unclear whether a subset of CTCF binding sites plays a functional role in establishing/maintaining chromatin topological domains.

Results

We systematically analysed the genomic, transcriptomic and epigenetic profiles of the CTCF binding sites in 56 human cell lines from ENCODE. We identified ~24,000 CTCF sites (referred to as constitutive sites) that were bound in more than 90% of the cell lines. Our analysis revealed: 1) constitutive CTCF

SUBMITTER: Li Y 

PROVIDER: S-EPMC3765723 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature