Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus.

ABSTRACT:

Background

Cotton leaf curl disease, caused by single-stranded DNA viruses of the genus Begomovirus (family Geminiviridae), is a major constraint to cotton cultivation across Pakistan and north-western India. At this time only cotton varieties with moderate tolerance are available to counter the disease. microRNAs (miRNAs) are a class of endogenous small RNA molecules that play an important role in plant development, signal transduction, and response to biotic and a biotic stress. Studies have shown that miRNAs can be engineered to alter their target specificity. Such artificial miRNAs (amiRNAs) have been shown to provide resistance against plant-infecting viruses.

Results

Two amiRNA constructs, based on the sequence of cotton miRNA169a, were produced containing 21 nt of the

SUBMITTER: Ali I 

PROVIDER: S-EPMC3765727 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature