Identification of a novel infection-enhancing epitope on dengue prM using a dengue cross-reacting monoclonal antibody.

ABSTRACT:

Background

Dengue virus (DENV) infection is the most important arthropod- borne viral disease in human, but antiviral therapy and approved vaccines remain unavailable due to antibody-dependent enhancement (ADE) phenomenon. Many studies showed that pre-membrane (prM)-specific antibodies do not efficiently neutralize DENV infection but potently promote ADE infection. However, most of the binding epitopes of these antibodies remain unknown.

Results

In the present study, we characterized a DENV cross-reactive monoclonal antibody (mAb), 4D10, that neutralized poorly but potently enhanced infection of four standard DENV serotypes and immature DENV (imDENV) over a broad range of concentration. In addition, the epitope of 4D10 was successfully mapped to amino acid residues 14 to18 o

SUBMITTER: Luo YY 

PROVIDER: S-EPMC3765915 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature