Cold shock Y-box protein-1 proteolysis autoregulates its transcriptional activities.

ABSTRACT:

Background

The Y-box protein-1 (YB-1) fulfills pleiotropic functions relating to gene transcription, mRNA processing, and translation. It remains elusive how YB-1 shuttling into the nuclear and cytoplasmic compartments is regulated and whether limited proteolysis by the 20S proteasome releases fragments with distinct function(s) and subcellular distribution(s).

Results

To address these questions, mapping of domains responsible for subcellular targeting was performed. Three nuclear localization signals (NLS) were identified. NLS-1 (aa 149-156) and NLS-2 (aa 185-194) correspond to residues with unknown function(s), whereas NLS-3 (aa 276-292) matches with a designated multimerization domain. Nuclear export signal(s) were not identified. Endoproteolytic processing by the 20S pro

SUBMITTER: van Roeyen CR 

PROVIDER: S-EPMC3766096 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature