A validated stability-indicating TLC-densitometric method for the determination of stanozolol in pharmaceutical formulations.

ABSTRACT:

Background

Stanozolol is a synthetic derivative of dihydrotestosterone (DHT), and one of the frequently detected anabolic steroids in doping analysis. The current work describes the development and validation of the stability-indicating TLC-densitometric method for sensitive and specific estimation of stanozolol even its degradation product being there. Precoated silica gel TLC aluminium plates were utilized as the stationary phase and the eluent comprised of petroleum ether: acetone (6:4, v/v). Densitometric analysis of stanozolol was achieved at λmax 750 nm in the absorbance mode after staining with phosphomolybdic acid (PMA). Stress degradation of stanozolol was carried out under various reaction conditions including acid, base and neutral hydrolysis, wet and dry heating treatme

SUBMITTER: Musharraf SG 

PROVIDER: S-EPMC3766227 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature