Spectral library generating function for assessing spectrum-spectrum match significance.

ABSTRACT: Tandem mass spectrometry (MS/MS) continues to be the technology of choice for high-throughput analysis of complex proteomics samples. While MS/MS spectra are commonly identified by matching against a database of known protein sequences, the complementary approach of spectral library searching against collections of reference spectra consistently outperforms sequence-based searches by resulting in significantly more identified spectra. However, while spectral library searches benefit from the advance knowledge of the expected peptide fragmentation patterns recorded in library spectra, estimation of the statistical significance of spectrum-spectrum matches (SSMs) continues to be hindered by difficulties in finding an appropriate definition of "random" SSMs to use as a null model when estimat

SUBMITTER: Wang M 

PROVIDER: S-EPMC3766387 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature