Multiphasic DNA adsorption to silica surfaces under varying buffer, pH, and ionic strength conditions.

ABSTRACT: Reversible interactions between DNA and silica are utilized in the solid phase extraction and purification of DNA from complex samples. Chaotropic salts commonly drive DNA binding to silica but inhibit DNA polymerase amplification. We studied DNA adsorption to silica using conditions with or without chaotropic salts through bulk depletion and quartz crystal microbalance (QCM) experiments. While more DNA adsorbed to silica using chaotropic salts, certain buffer conditions without chaotropic salts yielded a similar amount of eluted DNA. QCM results indicate that under stronger adsorbing conditions the adsorbed DNA layer is initially rigid but becomes viscoelastic within minutes. These results qualitatively agreed with a mathematical model for a multiphasic adsorption process. Buffer conditio

SUBMITTER: Vandeventer PE 

PROVIDER: S-EPMC3766398 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature