Molecular characterization and identification of members of the Anopheles subpictus complex in Sri Lanka.

ABSTRACT:

Background

Anopheles subpictus sensu lato is a major malaria vector in South and Southeast Asia. Based initially on polytene chromosome inversion polymorphism, and subsequently on morphological characterization, four sibling species A-D were reported from India. The present study uses molecular methods to further characterize and identify sibling species in Sri Lanka.

Methods

Mosquitoes from Sri Lanka were morphologically identified to species and sequenced for the ribosomal internal transcribed spacer-2 (ITS2) and the mitochondrial cytochrome c oxidase subunit-I (COI) genes. These sequences, together with others from GenBank, were used to construct phylogenetic trees and parsimony haplotype networks and to test for genetic population structure.

Results

Both ITS2 and

SUBMITTER: Surendran SN 

PROVIDER: S-EPMC3766661 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature