Pathogenesis and Chronologic Localization of the Human Influenza A (H1N1) Virus in Cotton Rats.

ABSTRACT:

Objectives

We aimed to evaluate the pathogenesis and chronologic localization of human influenza A (H1N1) virus in experimentally infected cotton rats.

Methods

The animals were intranasally inoculated with 10(7) plaque-forming units of A/Solomon Islands/3/2006 (H1N1) influenza virus and evaluated for pathogenicity for a period of 28 days. Virus replication kinetics and pathological properties were assessed chronologically. Acute antiviral responses were evaluated by mean of real-time polymerase chain reaction.

Results

Cotton rats infected with A/Solomon Islands/3/2006 virus lost weight until 6 days post-inoculation (DPI) and showed decreased activity until 3 DPI. At necropsy, focal areas of redness and consolidation of lungs were evident at 1, 2, and 3 DPI. Lung histo

SUBMITTER: Kwon D 

PROVIDER: S-EPMC3766908 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature