The first outbreak of giardiasis with drinking water in Korea.

ABSTRACT:

Objectives

To identify the pathogen of the diarrhea outbreak in a village in Jeollabuk province in Korea in April 2010.

Methods

DNA extraction was performed from the 120 L of collected water, which was centrifuged at 10,000 x g for 30 min. PCR reactions were conducted in a total of 25 ul, which included PCR premix (GenDEPOT, Barker, TX, USA), 2 ul (∼100 ng) of extracted DNA, and 10 pmol of each primer.

Results

Nine people out of 25 had a symptom of abdominal pain accompanied by diarrhea after they used stored valley water in a water tank as a provisional water supply source without chlorine sterilization. Among them Giardia lamblia was detected in fecal samples of 7 people using the polymerase chain reaction method. Although G. lamblia was also detected from water pro

SUBMITTER: Cheun HI 

PROVIDER: S-EPMC3767097 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature