Establishing a hematopoietic genetic network through locus-specific integration of chromatin regulators.

ABSTRACT: The establishment and maintenance of cell type-specific transcriptional programs require an ensemble of broadly expressed chromatin remodeling and modifying enzymes. Many questions remain unanswered regarding the contributions of these enzymes to specialized genetic networks that control critical processes, such as lineage commitment and cellular differentiation. We have been addressing this problem in the context of erythrocyte development driven by the transcription factor GATA-1 and its coregulator Friend of GATA-1 (FOG-1). As certain GATA-1 target genes have little to no FOG-1 requirement for expression, presumably additional coregulators can mediate GATA-1 function. Using a genetic complementation assay and RNA interference in GATA-1-null cells, we demonstrate a vital link between GAT

SUBMITTER: DeVilbiss AW 

PROVIDER: S-EPMC3767509 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature