Evaluation of inhibitor-resistant real-time PCR methods for diagnostics in clinical and environmental samples.

ABSTRACT: Polymerase chain reaction (PCR) is commonly used for pathogen detection in clinical and environmental samples. These sample matrices often contain inhibitors of PCR, which is a primary reason for sample processing; however, the purification process is highly inefficient, becoming unacceptable at lower signature concentrations. One potential solution is direct PCR assessment without sample processing. Here, we evaluated nine inhibitor-resistant PCR reagents for direct detection of Francisella tularensis in seven different clinical and environmental samples using an established real-time PCR assay to assess ability to overcome PCR inhibition. While several of these reagents were designed for standard PCR, the described inhibitor resistant properties (ex. Omni Klentaq can amplify target DNA s

SUBMITTER: Trombley Hall A 

PROVIDER: S-EPMC3767612 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature