Nanodiscs allow phage display selection for ligands to non-linear epitopes on membrane proteins.

ABSTRACT: In this work, we exploited a method that uses polytopic membrane proteins as targets for phage display selections. Membrane proteins represent the largest class of drug targets and drug discovery is mostly based on the identification of ligands binding to target molecules. The screening of a phage display library for ligands against membrane proteins is typically hindered by the requirement of these proteins for a membrane environment, which is necessary to retain correct folding and epitope formation. Especially in proteins with multiple transmembrane domains, epitopes often are non-linear and consist of a combination of loops between transmembrane stretches of the proteins. Here, we have used bacteriorhodopsin (bR) as a model of polytopic membrane protein, assembled into nanoscale phosph

SUBMITTER: Pavlidou M 

PROVIDER: S-EPMC3767683 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature