Keratin23 (KRT23) knockdown decreases proliferation and affects the DNA damage response of colon cancer cells.

ABSTRACT: Keratin 23 (KRT23) is strongly expressed in colon adenocarcinomas but absent in normal colon mucosa. Array based methylation profiling of 40 colon samples showed that the promoter of KRT23 was methylated in normal colon mucosa, while hypomethylated in most adenocarcinomas. Promoter methylation correlated with absent expression, while increased KRT23 expression in tumor samples correlated with promoter hypomethylation, as confirmed by bisulfite sequencing. Demethylation induced KRT23 expression in vitro. Expression profiling of shRNA mediated stable KRT23 knockdown in colon cancer cell lines showed that KRT23 depletion affected molecules of the cell cycle and DNA replication, recombination and repair. In vitro analyses confirmed that KRT23 depletion significantly decreased the cellular prol

SUBMITTER: Birkenkamp-Demtroder K 

PROVIDER: S-EPMC3767798 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature