Development of a polymerase chain reaction assay for the detection of pseudorabies virus in clinical samples.

ABSTRACT: Aujeszky's disease, also known as pseudorabies causes severe economic losses in swine industry and affects the pig husbandry all over the world. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. The development, optimization and evaluation of a polymerase chain reaction (PCR) assay are presented for the diagnosis of pseudorabies infection. This assay was based on the amplification of a highly conserved viral gD gene fragment. PCR products of the expected size were obtained from PRV strains. Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. The analytical sensitivity of the test was estimated to be 1.34 TC

SUBMITTER: Perez LJ 

PROVIDER: S-EPMC3768535 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature