Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.).

ABSTRACT: Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identif

SUBMITTER: Figueiredo JE 

PROVIDER: S-EPMC3768548 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature