A data-driven approach to preprocessing Illumina 450K methylation array data.

ABSTRACT:

Background

As the most stable and experimentally accessible epigenetic mark, DNA methylation is of great interest to the research community. The landscape of DNA methylation across tissues, through development and in disease pathogenesis is not yet well characterized. Thus there is a need for rapid and cost effective methods for assessing genome-wide levels of DNA methylation. The Illumina Infinium HumanMethylation450 (450K) BeadChip is a very useful addition to the available methods for DNA methylation analysis but its complex design, incorporating two different assay methods, requires careful consideration. Accordingly, several normalization schemes have been published. We have taken advantage of known DNA methylation patterns associated with genomic imprinting and X-chromosome i

SUBMITTER: Pidsley R 

PROVIDER: S-EPMC3769145 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature