Determination of dosage compensation of the mammalian X chromosome by RNA-seq is dependent on analytical approach.

ABSTRACT:

Background

An enduring question surrounding sex chromosome evolution is whether effective hemizygosity in the heterogametic sex leads inevitably to dosage compensation of sex-linked genes, and whether this compensation has been observed in a variety of organisms. Incongruence in the conclusions reached in some recent reports has been attributed to different high-throughput approaches to transcriptome analysis. However, recent reports each utilizing RNA-seq to gauge X-linked gene expression relative to autosomal gene expression also arrived at diametrically opposed conclusions regarding X chromosome dosage compensation in mammals.

Results

Here we analyze RNA-seq data from X-monosomic female human and mouse tissues, which are uncomplicated by genes that escape X-inactivation,

SUBMITTER: Jue NK 

PROVIDER: S-EPMC3769146 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature