Resequencing of Treponema pallidum ssp. pallidum strains Nichols and SS14: correction of sequencing errors resulted in increased separation of syphilis treponeme subclusters.

ABSTRACT:

Background

Treponema pallidum ssp. pallidum (TPA), the causative agent of syphilis, is a highly clonal bacterium showing minimal genetic variability in the genome sequence of individual strains. Nevertheless, genetically characterized syphilis strains can be clearly divided into two groups, Nichols-like strains and SS14-like strains. TPA Nichols and SS14 strains were completely sequenced in 1998 and 2008, respectively. Since publication of their complete genome sequences, a number of sequencing errors in each genome have been reported. Therefore, we have resequenced TPA Nichols and SS14 strains using next-generation sequencing techniques.

Methodology/principal findings

The genomes of TPA strains Nichols and SS14 were resequenced using the 454 and Illumina sequencing methods

SUBMITTER: Petrosova H 

PROVIDER: S-EPMC3769245 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature