Multicenter intestinal current measurements in rectal biopsies from CF and non-CF subjects to monitor CFTR function.

ABSTRACT: Intestinal current measurements (ICM) from rectal biopsies are a sensitive means to detect cystic fibrosis transmembrane conductance regulator (CFTR) function, but have not been optimized for multicenter use. We piloted multicenter standard operating procedures (SOPs) to detect CFTR activity by ICM and examined key questions for use in clinical trials. SOPs for ICM using human rectal biopsies were developed across three centers and used to characterize ion transport from non-CF and CF subjects (two severe CFTR mutations). All data were centrally evaluated by a blinded interpreter. SOPs were then used across four centers to examine the effect of cold storage on CFTR currents and compare CFTR currents in biopsies from one subject studied simultaneously either at two sites (24 hours post-biop

SUBMITTER: Clancy JP 

PROVIDER: S-EPMC3769519 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature