TreeParser-aided Klee diagrams display taxonomic clusters in DNA barcode and nuclear gene datasets.

ABSTRACT: Indicator vector analysis of a nucleotide sequence alignment generates a compact heat map, called a Klee diagram, with potential insight into clustering patterns in evolution. However, so far this approach has examined only mitochondrial cytochrome c oxidase I (COI) DNA barcode sequences. To further explore, we developed TreeParser, a freely-available web-based program that sorts a sequence alignment according to a phylogenetic tree generated from the dataset. We applied TreeParser to nuclear gene and COI barcode alignments from birds and butterflies. Distinct blocks in the resulting Klee diagrams corresponded to species and higher-level taxonomic divisions in both groups, and this enabled graphic comparison of phylogenetic information in nuclear and mitochondrial genes. Our results demons

SUBMITTER: Stoeckle MY 

PROVIDER: S-EPMC3769653 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature