Characterization of Mycobacterium tuberculosis complex isolated from iranian and afghani patients by spoligotyping method.

ABSTRACT: Designing newer drugs, vaccines, and diagnostic techniques is dependent on better understanding of M. tuberculosis virulence mechanism. In this study the prevalence of pcaA gene was determined in M. tuberculosis strains typed by spoligotyping. The associated risk factors among patients with different nationalities residing in Iran were also determined. The isolated M. tuberculosis strains have been characterized by performing susceptibility tests against four first-line antituberculosis drugs and were then subjected to spoligotyping characterization. PCR was used for detection of pcaA gene and its nucleotide sequence was also determined. Spoligotyping of M. tuberculosis strains resulted in 140 different patterns. One hundred twenty two (87.1%) of these spoligotype isolates were unique and

SUBMITTER: Ramazanzadeh R 

PROVIDER: S-EPMC3769729 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature