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Detection of Bartonella henselae in defibrinated sheep blood used for culture media supplementation.


ABSTRACT: Bartonella henselae was detected in defibrinated sheep blood employed in supplementing a selective bacteria culture medium by nested PCR. We recommended that highly sensitive technical tests be run to ensure a sterile culture medium for Bartonella spp. isolation, since infected blood samples used in preparation could lead to false-positive results.

SUBMITTER: Drummond MR 

PROVIDER: S-EPMC3769854 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Detection of Bartonella henselae in defibrinated sheep blood used for culture media supplementation.

Drummond Marina Rovani MR   Pitassi Luiza H U LH   Lania Bruno G BG   Dos Santos Silvio R C SR   Gilioli Rovilson R   Velho Paulo E N F PE  

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20110401 2


Bartonella henselae was detected in defibrinated sheep blood employed in supplementing a selective bacteria culture medium by nested PCR. We recommended that highly sensitive technical tests be run to ensure a sterile culture medium for Bartonella spp. isolation, since infected blood samples used in preparation could lead to false-positive results. ...[more]

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