Regulation of cardiac microRNAs by cardiac microRNAs.

ABSTRACT:

Rationale

MicroRNAs modestly suppress their direct mRNA targets, and these direct effects are amplified by modulation of gene transcription pathways. Consequently, indirect mRNA modulatory effects of microRNAs to increase or decrease mRNAs greatly outnumber direct target suppressions. Because microRNAs are products of transcription, the potential exists for microRNAs that regulate transcription to regulate other microRNAs.

Objective

Determine whether cardiac-expressed microRNAs regulate expression of other cardiac microRNAs, and measure the impact of microRNA-mediated microRNA regulation on indirect regulation of nontarget mRNAs.

Methods and results

Transgenic expression of pre-microRNAs was used to generate mouse hearts expressing 6- to 16-fold normal levels of micro

SUBMITTER: Matkovich SJ 

PROVIDER: S-EPMC3769967 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature