Comparative lipidomics of mouse brain exposed to enriched environment.

ABSTRACT: Several studies have shown that housing conditions and environmental exposure to a series of stimuli lead to behavior improvement in several species. While more works have been focused on illustrating changes of the proteome and transcriptome following enriched environment exposure in mice, little has been done to understand changes in the brain metabolome in this paradigm due to the complexity of this type of analysis. In this paper, lipidomics focused on phospholipids and gangliosides were conducted for brain tissues of mice exposed to enriched or impoverished conditions. We optimized previously reported method and established a reliable relative comparison method for phospholipids and gangliosides in brain tissue using prefractionation with weak anion exchange cartridge. We used liquid

SUBMITTER: Sato Y 

PROVIDER: S-EPMC3770082 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature