Long-range transcriptional regulation by the p110 CUX1 homeodomain protein on the ENCODE array.

ABSTRACT:

Background

Overexpression of the Cut homeobox 1 gene, CUX1, inversely correlates with patient survival in breast cancers. Cell-based assays and molecular studies have revealed that transcriptional regulation by CUX1 involves mostly the proteolytically processed p110 isoform. As there is no antibody specific to p110 CUX1 only, an alternate strategy must be employed to identify its targets.

Results

We expressed physiological levels of a tagged-p110 CUX1 protein and performed chromatin affinity purification followed by hybridization on ENCODE and promoter arrays. Targets were validated by chromatin immunoprecipitation and transcriptional regulation by CUX1 was analyzed in expression profiling and RT-qPCR assays following CUX1 knockdown or p110 CUX1 overexpression. Approximately

SUBMITTER: Vadnais C 

PROVIDER: S-EPMC3770232 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature