Determination of sRNA expressions by RNA-seq in Yersinia pestis grown in vitro and during infection.

ABSTRACT:

Background

Small non-coding RNAs (sRNAs) facilitate host-microbe interactions. They have a central function in the post-transcriptional regulation during pathogenic lifestyles. Hfq, an RNA-binding protein that many sRNAs act in conjunction with, is required for Y. pestis pathogenesis. However, information on how Yersinia pestis modulates the expression of sRNAs during infection is largely unknown.

Methodology and principal findings

We used RNA-seq technology to identify the sRNA candidates expressed from Y. pestis grown in vitro and in the infected lungs of mice. A total of 104 sRNAs were found, including 26 previously annotated sRNAs, by searching against the Rfam database with 78 novel sRNA candidates. Approximately 89% (93/104) of these sRNAs from Y. pestis are shared wit

SUBMITTER: Yan Y 

PROVIDER: S-EPMC3770706 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature