Characterization of mRNA-cytoskeleton interactions in situ using FMTRIP and proximity ligation.

ABSTRACT: Many studies have demonstrated an association between the cytoskeleton and mRNA, as well as the asymmetric distribution of mRNA granules within the cell in response to various signaling events. It is likely that the extensive cytoskeletal network directs mRNA transport and localization, with different cytoskeletal elements having their own specific roles. In order to understand the spatiotemporal changes in the interactions between the mRNA and the cytoskeleton as a response to a stimulus, a technique that can visualize and quantify these changes across a population of cells while capturing cell-to-cell variations is required. Here, we demonstrate a method for imaging and quantifying mRNA-cytoskeleton interactions on a per cell basis with single-interaction sensitivity. Using a proximity l

SUBMITTER: Jung J 

PROVIDER: S-EPMC3770708 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature