Molecular control of the amount, subcellular location, and activity state of translation elongation factor 2 in neurons experiencing stress.

ABSTRACT: Eukaryotic elongation factor 2 (eEF-2) is an important regulator of the protein translation machinery whereby it controls the movement of the ribosome along the mRNA. The activity of eEF-2 is regulated by changes in cellular energy status and nutrient availability and by posttranslational modifications such as phosphorylation and mono-ADP-ribosylation. However, the mechanisms regulating protein translation under conditions of cellular stress in neurons are unknown. Here we show that when rat hippocampal neurons experience oxidative stress (lipid peroxidation induced by exposure to cumene hydroperoxide; CH), eEF-2 is hyperphosphorylated and ribosylated, resulting in reduced translational activity. The degradation of eEF-2 requires calpain proteolytic activity and is accompanied by accumulat

SUBMITTER: Arguelles S 

PROVIDER: S-EPMC3772990 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature