Porphyrin π-stacking in a heme protein scaffold tunes gas ligand affinity.

ABSTRACT: The role of π-stacking in controlling redox and ligand binding properties of porphyrins has been of interest for many years. The recent discovery of H-NOX domains has provided a model system to investigate the role of porphyrin π-stacking within a heme protein scaffold. Removal of a phenylalanine-porphyrin π-stack dramatically increased O2, NO, and CO affinities and caused changes in redox potential (~40mV) without any structural changes. These results suggest that small changes in redox potential affect ligand affinity and that π-stacking may provide a novel route to engineer heme protein properties for new functions.

SUBMITTER: Weinert EE 

PROVIDER: S-EPMC3773300 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature