A novel method to study contact inhibition of locomotion using micropatterned substrates.

ABSTRACT: The concept of contact inhibition of locomotion (CIL) describes the ability of a cell to change the direction of its movement after contact with another cell. It has been shown to be responsible for physiological and developmental processes such as wound healing, macrophage dispersion and neural crest cell migration; whereas its loss facilitates cancer cell invasion and metastatic dissemination. Different assays have been developed to analyze CIL in tissue culture models. However, these methods have several caveats. Collisions happen at low frequency between freely migrating cells and the orientation of the cells at the time of contact is not predictable. Moreover, the computational analysis required by these assays is often complicated and it retains a certain degree of discretion. Here,

SUBMITTER: Scarpa E 

PROVIDER: S-EPMC3773336 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature