Excitatory input onto hilar somatostatin interneurons is increased in a chronic model of epilepsy.

ABSTRACT: The density of somatostatin (SOM)-containing GABAergic interneurons in the hilus of the dentate gyrus is significantly decreased in both human and experimental temporal lobe epilepsy. We used the pilocarpine model of status epilepticus and temporal lobe epilepsy in mice to study anatomical and electrophysiological properties of surviving somatostatin interneurons and determine whether compensatory functional changes occur that might offset loss of other inhibitory neurons. Using standard patch-clamp techniques and pipettes containing biocytin, whole cell recordings were obtained in hippocampal slices maintained in vitro. Hilar SOM cells containing enhanced green fluorescent protein (EGFP) were identified with fluorescent and infrared differential interference contrast video microscopy in e

SUBMITTER: Halabisky B 

PROVIDER: S-EPMC3774571 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature