Genome-wide shRNA screening to identify factors mediating Gata6 repression in mouse embryonic stem cells.

ABSTRACT: The use of whole-genome pooled shRNA libraries in loss-of-function screening in tissue culture models provides an effective means to identify novel factors acting in pathways of interest. Embryonic stem cells (ESCs) offer a unique opportunity to study processes involved in stem cell pluripotency and differentiation. Here, we report a genome-wide shRNA screen in ESCs to identify novel components involved in repression of the Gata6 locus, using a cell viability-based screen, which offers the benefits of stable shRNA integration and a robust and simple protocol for hit identification. Candidate factors identified were enriched for transcription factors and included known Polycomb proteins and other chromatin-modifying factors. We identified the protein Bcor, which is known to associate in com

SUBMITTER: Cooper S 

PROVIDER: S-EPMC3775421 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature