A fluorescence-based assay for p38α recruitment site binders: identification of rooperol as a novel p38α kinase inhibitor.

ABSTRACT: A new p38α inhibitor: Using a D-recruitment site (DRS) probe for p38α which exploits covalent interaction with Cys119 and alkyne-azide "click" chemistry to identify small molecules that recognize the p38α DRS, the anti-inflammatory natural product rooperol was identified as a novel p38α inhibitor.

SUBMITTER: Li J 

PROVIDER: S-EPMC3775607 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature