Unraveling the role of KIAA1199, a novel endoplasmic reticulum protein, in cancer cell migration.

ABSTRACT:

Background

Cell migration is a critical determinant of cancer metastasis, and a better understanding of the genes involved will lead to the identification of novel targets aimed at preventing cancer dissemination. KIAA1199 has been shown to be upregulated in human cancers, yet its role in cancer progression was hitherto unknown.

Methods

Clinical relevance was assessed by examining KIAA1199 expression in human cancer specimens. In vitro and in vivo studies were employed to determine the function of KIAA1199 in cancer progression. Cellular localization of KIAA1199 was microscopically determined. SNAP-tag pull-down assays were used to identify binding partner(s) of KIAA1199. Calcium levels were evaluated using spectrofluorometric and fluorescence resonance energy transfer analy

SUBMITTER: Evensen NA 

PROVIDER: S-EPMC3776264 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature