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Construction of a thiamin sensor from the periplasmic thiamin binding protein.

ABSTRACT: This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity.

SUBMITTER: Hanes JW 

PROVIDER: S-EPMC3776480 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Construction of a thiamin sensor from the periplasmic thiamin binding protein.

Hanes Jeremiah W JW   Chatterjee Debashree D   Soriano Erika V EV   Ealick Steven E SE   Begley Tadhg P TP  

Chemical communications (Cambridge, England) 20101202 8

This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity. ...[more]

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