Epigenetic regulation of Sox4 during palate development.

ABSTRACT:

Aim

Identification of genes that contribute to secondary palate development provide a better understanding of the etiology of palatal clefts. Gene-expression profiling of the murine palate from gestational days 12-14 (GD12-14), a critical period in palate development, identified Sox4 as a differentially expressed gene. In this study, we have examined if the differential expression of Sox4 in the palate is due to changes in DNA methylation.

Materials & methods

In situ hybridization analysis was used to localize the expression of Sox4 in the developing murine secondary palate. CpG methylation profiling of a 1.8-kb upstream region of Sox4 in the secondary palate from GD12-14 and transfection analysis in murine embryonic maxillary mesenchymal cells using Sox4 deletion, mutant an

SUBMITTER: Seelan RS 

PROVIDER: S-EPMC3776497 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature