Identification of reference genes for qRT-PCR analysis in Yesso scallop Patinopecten yessoensis.

ABSTRACT:

Background

Bivalves comprise around 30,000 extant species and have received much attention for their importance in ecosystems, aquaculture and evolutionary studies. Despite the increasing application of real-time quantitative reverse transcription PCR (qRT-PCR) in gene expression studies on bivalve species, little research has been conducted on reference gene selection which is critical for reliable and accurate qRT-PCR analysis. For scallops, systematic evaluation of reference genes that can be used among tissues or embryo/larva stages is lacking, and β-actin (ACT) is most frequently used as qRT-PCR reference gene without validation.

Results

In this study, 12 commonly used candidate reference genes were selected from the transcriptome data of Yesso scallop (Patinopectenyess

SUBMITTER: Feng L 

PROVIDER: S-EPMC3777977 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature