Gene expression profiling using nanostring digital RNA counting to identify potential target antigens for melanoma immunotherapy.

ABSTRACT:

Purpose

The success of immunotherapy for the treatment of metastatic cancer is contingent on the identification of appropriate target antigens. Potential targets must be expressed on tumors but show restricted expression on normal tissues. To maximize patient eligibility, ideal target antigens should be expressed on a high percentage of tumors within a histology and, potentially, in multiple different malignancies.

Design

A Nanostring probeset was designed containing 97 genes, 72 of which are considered potential candidate genes for immunotherapy. Five established melanoma cell lines, 59 resected metastatic melanoma tumors, and 31 normal tissue samples were profiled and analyzed using Nanostring technology.

Results

Of the 72 potential target genes, 33 were overexpress

SUBMITTER: Beard RE 

PROVIDER: S-EPMC3778100 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature