Genome signature-based dissection of human gut metagenomes to extract subliminal viral sequences.

ABSTRACT: Bacterial viruses (bacteriophages) have a key role in shaping the development and functional outputs of host microbiomes. Although metagenomic approaches have greatly expanded our understanding of the prokaryotic virosphere, additional tools are required for the phage-oriented dissection of metagenomic data sets, and host-range affiliation of recovered sequences. Here we demonstrate the application of a genome signature-based approach to interrogate conventional whole-community metagenomes and access subliminal, phylogenetically targeted, phage sequences present within. We describe a portion of the biological dark matter extant in the human gut virome, and bring to light a population of potentially gut-specific Bacteroidales-like phage, poorly represented in existing virus like particle-de

SUBMITTER: Ogilvie LA 

PROVIDER: S-EPMC3778543 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature