Clonal genetic and hematopoietic heterogeneity among human-induced pluripotent stem cell lines.

ABSTRACT: Induced pluripotent stem cells (iPSCs) hold great promise for modeling human hematopoietic diseases. However, intrinsic variability in the capacities of different iPSC lines for hematopoietic development complicates comparative studies and is currently unexplained. We created and analyzed 3 separate iPSC clones from fibroblasts of 3 different normal individuals using a standardized approach that included excision of integrated reprogramming genes by Cre-Lox mediated recombination. Gene expression profiling and hematopoietic differentiation assays showed that independent lines from the same individual were generally more similar to one another than those from different individuals. However, one iPSC line (WT2.1) exhibited a distinctly different gene expression, proliferation rate, and hemat

SUBMITTER: Mills JA 

PROVIDER: S-EPMC3778548 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature