Mitochondrial protein ATPase family, AAA domain containing 3A correlates with radioresistance in glioblastoma.

ABSTRACT:

Background

ATPase-family, AAA domain containing 3A (ATAD3A) is located on human chromosome 1p36.33, and high endogenous expression may associate with radio- and chemosensitivity. This study was conducted to investigate the significance of ATAD3A in glioblastoma multiforme (GBM).

Methods

Clinical significance of ATAD3A expression was assessed by immunohistochemistry in 67 GBM specimens, and prognostic value was assessed in 32 GBM patients statistically. To investigate in vitro phenotypic effects of ATAD3A, cell viability was measured using a clonogenic survival assay under either knockdown or ectopic expression of ATAD3A in GBM cell lines. The effects of ATAD3A knockdown on targeted DNA repair-associated proteins in T98G cells were evaluated using immunofluorescence and Weste

SUBMITTER: You WC 

PROVIDER: S-EPMC3779036 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature