Expression, surface immobilization, and characterization of functional recombinant cannabinoid receptor CB2.

ABSTRACT: Human peripheral cannabinoid receptor CB2, a G protein-coupled receptor (GPCR) involved in regulation of immune response has become an important target for pharmaceutical drug development. Structural and functional studies on CB2 may benefit from immobilization of the purified and functional receptor onto a suitable surface at a controlled density and, preferably in a uniform orientation. The goal of this project was to develop a generic strategy for preparation of functional recombinant CB2 and immobilization at solid interfaces. Expression of CB2 as a fusion with Rho-tag (peptide composed of the last nine amino acids of rhodopsin) in E. coli was evaluated in terms of protein levels, accessibility of the tag, and activity of the receptor. The structural integrity of CB2 was tested by liga

SUBMITTER: Locatelli-Hoops SC 

PROVIDER: S-EPMC3779079 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature