Diagnosis of tumors during tissue-conserving surgery with integrated autofluorescence and Raman scattering microscopy.

ABSTRACT: Tissue-conserving surgery is used increasingly in cancer treatment. However, one of the main challenges in this type of surgery is the detection of tumor margins. Histopathology based on tissue sectioning and staining has been the gold standard for cancer diagnosis for more than a century. However, its use during tissue-conserving surgery is limited by time-consuming tissue preparation steps (1-2 h) and the diagnostic variability inherent in subjective image interpretation. Here, we demonstrate an integrated optical technique based on tissue autofluorescence imaging (high sensitivity and high speed but low specificity) and Raman scattering (high sensitivity and high specificity but low speed) that can overcome these limitations. Automated segmentation of autofluorescence images was used to

SUBMITTER: Kong K 

PROVIDER: S-EPMC3780864 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature