Genomic insights into triple-negative and HER2-positive breast cancers using isogenic model systems.

ABSTRACT:

Introduction

In general, genomic signatures of breast cancer subtypes have little or no overlap owing to the heterogeneous genetic backgrounds of study samples. Thus, obtaining a reliable signature in the context of isogenic nature of the cells has been challenging and the precise contribution of isogenic triple negative breast cancer (TNBC) versus non-TNBC remains poorly defined.

Methods

We established isogenic stable cell lines representing TNBC and Human Epidermal Growth Factor Receptor 2 positive (HER2+) breast cancers by introducing HER2 in TNBC cell lines MDA-MB-231 and MDA-MB-468. We examined protein level expression and functionality of the transfected receptor by treatment with an antagonist of HER2. Using microarray profiling, we obtained a comprehensive gene list

SUBMITTER: Mudvari P 

PROVIDER: S-EPMC3781103 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature