Bi-PROF: bisulfite profiling of target regions using 454 GS FLX Titanium technology.

ABSTRACT: The use of next generation sequencing has expanded our view on whole mammalian methylome patterns. In particular, it provides a genome-wide insight of local DNA methylation diversity at single nucleotide level and enables the examination of single chromosome sequence sections at a sufficient statistical power. We describe a bisulfite-based sequence profiling pipeline, Bi-PROF, which is based on the 454 GS-FLX Titanium technology that allows to obtain up to one million sequence stretches at single base pair resolution without laborious subcloning. To illustrate the performance of the experimental workflow connected to a bioinformatics program pipeline (BiQ Analyzer HT) we present a test analysis set of 68 different epigenetic marker regions (amplicons) in five individual patient-derived xen

SUBMITTER: Gries J 

PROVIDER: S-EPMC3781196 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature